Signalling

Part:BBa_K176224:Design

Designed by: Danqian Liu, Zongxiao He, Hao Jiang   Group: iGEM09_USTC   (2009-10-10)


PoPS->mRFP->CI Inverter(cI-LVA+pCI)->luxI-LVA->PoPS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2382
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 573
    Illegal AgeI site found at 685
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Input: PoPS

Output1: mRFP

Output2: LuxI-LVA

Output3: PoPS

Design:

Input PoPS generates Output1 mRFP and CI-LVA

pCI generates Output2 LuxI-LVA and Output3 PoPS, repressed by CI-LVA


Source

I13502 K176189

References